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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is noticed in several tumors, which ends up in chromosome instability, and so might lead to tumorigenesis. This protein was uncovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a critical job in promoting mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been described. Two more splice variants were being noted but thorough nucleotide sequence information and facts is not however obtainable.
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Blocking peptides are peptides that bind specially on the concentrate on antibody and block antibody binding. These peptide commonly includes the epitope recognized from the antibody.
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This guide outlines further more attainable optimization of mobile and immunoassay parameters to guarantee the best possible benefits are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind on the epitope about the click here goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.
Focus on Facts Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation styles which contribute towards the temporal coordination of each mitotic event. Cyclin E sorts a fancy with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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